Journal: Cytotechnology

Article Title: Antidiabetic effect of green rooibos ( Aspalathus linearis ) extract in cultured cells and type 2 diabetic model KK-A y mice

doi: 10.1007/s10616-014-9816-y

Figure Lengend Snippet: Effect of GRE and aspalathin on glucose uptake, AMPK and Akt phosphorylation, and GLUT4 translocation in L6 myotubes. a–c Glucose uptake assay was conducted without or with GRE (0–800 µg/ml) or aspalathin (50 µM) and compound C (10 µM). Each value represents the mean ± SEM for 6 wells. Values not sharing a common letter are significantly different at P < 0.05 by Tukey–Kramer multiple comparisons test. d–f Western blotting analysis for AMPK and Akt phosphorylation and translocation of GLUT4 to the plasma membrane. Western blotting was conducted with anti-phospho-AMPK, anti-AMPK, anti-phopho-Akt, anti-Akt, anti-GLUT4 and anti-Na+K+-ATPase antibodies. Na+K+-ATPase is a marker enzyme of plasma membrane. PM plasma membrane fraction; Post-PM post-plasma membrane fraction. The bar diagramme in f shows the GLUT4/Na+K+-ATPase ratio of PM fraction to demonstrate the translocation of GLUT4 to the plasma membrane

Article Snippet: Keywords: AMPK, GLUT4, Hyperglycemia, L6 myotube, RIN-5F cell, Rooibos

Techniques: Phospho-proteomics, Translocation Assay, Western Blot, Clinical Proteomics, Membrane, Marker

Journal:

Article Title: Type III InsP 3 receptor channel stays open in the presence of increased calcium

doi: 10.1038/23954

Figure Lengend Snippet: Ca 2+ transients in RIN-5F cells after stimulation by ATP

Article Snippet: We thank E. Kaftan for all his assistance during the execution of these experiments, J. Putney for suggesting RIN-5F cells as source of type III InsP 3 R, and J.

Techniques: